Boster Immunoleader

antibody

Anti-TRAF2 Antibody

PB9516

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-TRAF2 Antibody

PB9516

Polyclonal

Rabbit IgG polyclonal antibody for TNF receptor-associated factor 2(TRAF2) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF2 (308-339aa RLDQDKIEALSSKVQQLERSIGLKDLAMADLE), different from the related mouse sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

TRAF2 (TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al. (1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al. (2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.

1. Aizawa, S., Nakano, H., Ishida, T., Horie, R., Nagai, M., Ito, K., Yagita, H., Okumura, K., Inoue, J., Watanabe, T. Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NF-kappa-B activation. J. Biol. Chem. 272: 2042-2045, 1997. 2. Kanamori, M., Suzuki, H., Saito, R., Muramatsu, M., Hayashizaki, Y. T2BP, a novel TRAF2 binding protein, can activate NF-kappa-B and AP-1 without TNF stimulation. Biochem. Biophys. Res. Commun. 290: 1108-1113, 2002. 3. Rothe, M., Xiong, J., Shu, H.-B., Williamson, K., Goddard, A., Goeddel, D. V. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Nat. Acad. Sci. 93: 8241-8246, 1996.

TRAF2

TNF receptor-associated factor 2

TNF receptor-associated factor 2

E3 ubiquitin-protein ligase TRAF2 antibody MGC:45012 antibody OTTHUMP00000022625 antibody OTTHUMP00000064745 antibody TNF receptor associated factor 2 antibody TNF receptor-associated factor 2 antibody TNF receptor-associated protein antibody TRAF 2 antibody TRAF2 antibody TRAF2_HUMAN antibody TRAP 3 antibody TRAP antibody TRAP3 antibody Tumor necrosis factor type 2 receptor associated protein 3 antibody Tumor necrosis factor type 2 receptor-associated protein 3 antibody

Q12933