Boster Immunoleader

antibody

Anti-CTCF Antibody

PB9493

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-CTCF Antibody

PB9493

Polyclonal

Rabbit IgG polyclonal antibody for Transcriptional repressor CTCF(CTCF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CTCF recombinant protein (Position: K521-R727). Human CTCF shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat CTCF, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

1. Chaumeil J, Skok JA (April 2012). "The role of CTCF in regulating V(D)J recombination". Curr. Opin. Immunol. 24(2): 153–9. 2. Filippova GN, Fagerlie S, Klenova EM, Myers C, Dehner Y, Goodwin G, Neiman PE, Collins SJ, Lobanenkov VV (June 1996). "An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes". Mol. Cell. Biol. 16 (6): 2802–13. 3. Phillips JE, Corces VG (June 2009)."CTCF: master weaver of the genome".Cell 137 (7): 1194–211.

CTCF

Transcriptional repressor CTCF

CCCTC-binding factor (zinc finger protein)

11 zinc finger protein antibody 11 zinc finger transcriptional repressor antibody 11-zinc finger protein antibody CCCTC binding factor (zinc finger protein) antibody CCCTC binding factor antibody CCCTC-binding factor antibody Ctcf antibody CTCF_HUMAN antibody CTCFL paralog antibody Transcriptional repressor CTCF antibody

P49711