Boster Immunoleader

antibody

Anti-Cdc25B Antibody

PB9488

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Cdc25B Antibody

PB9488

Polyclonal

Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 2(CDC25B) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat Predicted to work with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

1. Bulavin, D. V., Higashimoto, Y., Popoff, I. J., Gaarde, W. A., Basrur, V., Potapova, O., Appella, E., Fornace, A. J., Jr.Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase.Nature 411: 102-107, 2001. 2. Lane, S. A., Baker, E., Sutherland, G. R., Tonks, I., Hayward, N., Ellem, K.The human cell cycle gene CDC25B is located at 20p13.Genomics 15: 693-694, 1993.

CDC25B

M-phase inducer phosphatase 2

cell division cycle 25 homolog B (S. pombe)

AI604853 antibody Cdc 25B antibody Cdc25b antibody CDC25B1 antibody CDC25B2 antibody CDC25B3 antibody CDC25HU2 antibody Cdc25m2 antibody Cell division cycle 25 homolog B antibody Cell division cycle 25B antibody Cell division cycle 25B isoform 1 antibody Cell division cycle 25B isoform 2 antibody Cell division cycle 25B isoform 3 antibody Cell division cycle 25B isoform 4 antibody Dual specificity phosphatase Cdc25B antibody EC 3.1.3.48 antibody M phase inducer phosphatase 2 antibody M-phase inducer phosphatase 2 antibody MPIP2_HUMAN antibody

P30305