product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-Wnt2b Antibody
catalog :
PB9462
quantity :
100 ug/vial
price :
240 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
product information
Product Name :
Anti-Wnt2b Antibody
Catalog Number :
PB9462
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Protein Wnt-2b(WNT2B) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Price :
240 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human, mouse, rat
Applications :
WB,IHC-P
Western blot :
Concentration: 0.1-0.5μg/ml; Tested Species: Human
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat
Notes :
WB: The detection limit for Wnt2b is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2b (87-112 aa QRYPDIMRSVGEGAREWIRECQHQFR), identical to the related mouse and rat sequences.
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Background :
WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
Reference :
1. Katoh, M., Hirai, M., Sugimura, T., Terada, M. Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family. Oncogene 13: 873-876, 1996. 2. Ober, E. A., Verkade, H., Field, H. A., Stainier, D. Y. R. Mesodermal Wnt2b signalling positively regulates liver specification. Nature 442: 688-691, 2006. 3. Wolda, S. L., Moon, R. T. Cloning and developmental expression in Xenopus laevis of seven additional members of the Wnt family. Oncogene 7: 1941-1947, 1992.
Gene Name :
WNT2B
Protein Name :
Protein Wnt-2b
Gene Full Name :
wingless-type MMTV integration site family, member 2B
Synonyms :
Protein Wnt-13 antibody Protein Wnt-2b antibody Wingless type MMTV integration site family, member 13 antibody Wingless type MMTV integration site family, member 2B antibody WNT13 antibody Wnt2b antibody WNT2B_HUMAN antibody XWNT2 antibody XWNT2, Xenopus, homolog of antibody
Uniprot ID :
Q93097
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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