Anti-WASP Antibody | Boster Immunoleader PB9459 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-WASP Antibody

catalog :

PB9459

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Product Name :

Anti-WASP Antibody

Catalog Number :

PB9459

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Wiskott-Aldrich syndrome protein(WAS) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for WASP is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human WASP (129-156aa ADEDEAQAFRALVQEKIQKRNQRQSGDR), different from the related mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

Reference :

1. Jin, Y.; Mazza, C.; Christie, J.; Giliani, S.; Fiorini, M.; Mella, P.; Gandellini, F.; Stewart, D.; Zhu, Q.; Nelson, D. L.; Notarangelo, L. D.; Ochs, H. D. (2004). "Mutations of the Wiskott-Aldrich Syndrome Protein (WASP): hotspots, effect on transcription, and translation and phenotype/genotype correlation". Blood104 (13): 4010–4019. 2. Radl, J.; Dooren, L. H.; Morell, A.; Skvaril, F.; Vossen, J. M.; Uittenbogaart, C. H. (1976). "Immunoglobulins and transient paraproteins in sera of patients with the Wiskott-Aldrich syndrome: a follow-up study". Clinical and experimental immunology 25 (2): 256–263.

Gene Name :

WAS

Protein Name :

Wiskott-Aldrich syndrome protein

Gene Full Name :

Wiskott-Aldrich syndrome

Synonyms :

Eczema thrombocytopenia antibody IMD2 antibody SCNX antibody THC antibody THC1 antibody Thrombocytopenia 1 (X linked) antibody U42471 antibody Was antibody WAS protein antibody WASp antibody WASP_HUMAN antibody Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody Wiskott Aldrich syndrome (eczema thrombocytopenia) protein antibody Wiskott Aldrich syndrome antibody Wiskott Aldrich syndrome protein antibody Wiskott-Aldrich syndrome protein antibody

Uniprot ID :

P42768

more info or order :

Boster Immunoleader product webpage