Anti-TLR5 Antibody | Boster Immunoleader PB9451 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-TLR5 Antibody

catalog :

PB9451

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-TLR5 Antibody

Catalog Number :

PB9451

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Toll-like receptor 5(TLR5) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for TLR5 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human TLR5 (801-828aa MKHQSIRGFVQKQQYLRWPEDFQDVGWF), different from the related mouse sequence by five amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

TLR5 encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Reference :

1. "Entrez Gene: TLR5 toll-like receptor 5". 2. Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, Goodlett DR, Eng JK, Akira S, Underhill DM, Aderem A (April 2001). "The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5". Nature 410 (6832): 1099–1103. 3. Smith KD, Andersen-Nissen E, Hayashi F, Strobe K, Bergman MA, Barrett SL, Cookson BT, Aderem A (December 2003). "Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility". Nature Immunology 4 (12): 1247–53.

Gene Name :

TLR5

Protein Name :

Toll-like receptor 5

Gene Full Name :

toll-like receptor 5

Synonyms :

FLJ10052 antibody MGC126430 antibody MGC126431 antibody SLEB1 antibody TIL 3 antibody TIL3 antibody TLR 5 antibody Tlr5 antibody TLR5_HUMAN antibody Toll like receptor 5 antibody Toll like receptor 5 precursor antibody Toll-like receptor 5 antibody Toll/interleukin 1 receptor like protein 3 antibody Toll/interleukin-1 receptor-like protein 3 antibody

Uniprot ID :

O60602

more info or order :

Boster Immunoleader product webpage