Anti-Band 3 Antibody | Boster Immunoleader PB9437 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Band 3 Antibody

catalog :

PB9437

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Product Name :

Anti-Band 3 Antibody

Catalog Number :

PB9437

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Band 3 anion transport protein(SLC4A1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E. coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

Reference :

1. Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT et al. (1991). "Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis".Proc. Natl. Acad. Sci. U.S.A. 88 (24): 11022–6. 2. Schlüter K, Drenckhahn D (August 1986)."Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes". Proc. Natl. Acad. Sci. U.S.A. 83 (16): 6137–41.

Gene Name :

SLC4A1

Protein Name :

Band 3 anion transport protein

Gene Full Name :

solute carrier family 4 (anion exchanger), member 1 (Diego blood group)

Synonyms :

AE 1 antibody AE1 antibody Anion exchange protein 1 antibody Anion exchanger 1 antibody B3AT_HUMAN antibody Band 3 anion transport protein antibody BND3 antibody CD233 antibody DI antibody Diego blood group antibody EMPB3 antibody EPB3 antibody Erythrocyte membrane protein band 3 antibody Erythroid anion exchange protein antibody FR antibody Froese blood group antibody RTA1A antibody SLC4A1 antibody Solute carrier family 4 anion exchanger member 1 antibody Solute carrier family 4 member 1 antibody SW antibody Swann blood group antibody Waldner blood group antibody WD antibody WD1 antibody WR antibody Wright blood group antibody

Uniprot ID :

P02730

more info or order :

Boster Immunoleader product webpage