Boster Immunoleader

antibody

Anti-SF2 Antibody

PB9404

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-SF2 Antibody

PB9404

Polyclonal

Rabbit IgG polyclonal antibody for Serine/arginine-rich splicing factor 1(SRSF1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for SF2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2(6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE), identical to the related mouse sequence.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.

1. "Entrez Gene: SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor)". 2. Michlewski G, Sanford JR, Cáceres JF (April 2008). "The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1". Mol. Cell 30 (2): 179–89. 3. Zuo P, Manley JL (December 1993)."Functional domains of the human splicing factor ASF/SF2". EMBO J. 12 (12): 4727–37.

SRSF1

Serine/arginine-rich splicing factor 1

serine/arginine-rich splicing factor 1

Alternative splicing factor 1 antibody Alternative-splicing factor 1 antibody arginine/serine-rich 1 antibody ASF 1 antibody ASF antibody ASF-1 antibody ASF1 antibody FLJ53078 antibody MGC5228 antibody P33 subunit antibody Pre mRNA splicing factor SF2 P33 subunit antibody pre-mRNA-splicing factor SF2 antibody Serine/arginine-rich splicing factor 1 antibody SF2 antibody SF2P33 antibody SFRS1 antibody Splicing factor 2 alternate splicing factor antibody Splicing factor 2 antibody Splicing factor antibody Splicing factor arginine/serine rich 1 antibody SR Splicing factor 1 antibody SRp30a antibody srsf1 antibody SRSF1_HUMAN antibody

Q07955