Boster Immunoleader

antibody

Anti-ROCK2 Antibody

PB9387

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-ROCK2 Antibody

PB9387

Polyclonal

Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for ROCK2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

1. Takahashi, N.; Tuiki, H.; Saya, H.; Kaibuchi, K. : Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2. Thumkeo, D.; Keel, J.; Ishizaki, T.; Hirose, M.; Nonomura, K.; Oshima, H.; Oshima, M.; Taketo, M. M.; Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003.

ROCK2

Rho-associated protein kinase 2

Rho-associated, coiled-coil containing protein kinase 2

coiled-coil-containing protein kinase 2 antibody KIAA0619 antibody p164 ROCK 2 antibody p164 ROCK-2 antibody Rho associated coiled coil containing protein kinase 2 antibody Rho associated protein kinase 2 antibody Rho associated, coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody ROCK 2 antibody Rock II antibody Rock2 antibody ROCK2_HUMAN antibody Rock2m antibody ROK alpha antibody ROKalpha antibody

O75116