Anti-Calcineurin A Antibody | Boster Immunoleader PB9382 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Calcineurin A Antibody

catalog :

PB9382

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

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product information

Product Name :

Anti-Calcineurin A Antibody

Catalog Number :

PB9382

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform(PPP3CA) detection. Tested with WB in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Calcineurin A (488-521aa DAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.

Reference :

1. Musaro, A., McCullagh, K. J. A., Naya, F. J., Olson, E. N., Rosenthal, N. IGF-1 induces skeletal myocyte hypertrophy through calcineurin in association with GATA-2 and NF-ATc1. Nature 400: 581-585, 1999. Semsarian, C., Wu, M.-J., Ju, Y.-K., Marciniec, T., Yeoh, T., Allen, D. G., Harvey, R. P., Graham, R. M. Skeletal muscle hypertrophy is mediated by a Ca(2+)-dependent calcineurin signalling pathway. Nature 400: 576-581, 1999.

Gene Name :

PPP3CA

Protein Name :

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Gene Full Name :

protein phosphatase 3, catalytic subunit, alpha isozyme

Synonyms :

Alpha isoform formerly PPP2B antibody Calcineurin A alpha antibody Calcineurin A1 antibody CalcineurinA antibody Calmodulin dependent calcineurin A subunit alpha isoform antibody Calmodulin-dependent calcineurin A subunit alpha isoform antibody CALN antibody CALNA 1 antibody CALNA antibody CALNA1 antibody CAM PRP catalytic subunit antibody CAM-PRP catalytic subunit antibody CCN 1 antibody CCN1 antibody CNA 1 antibody CNA alpha antibody CNA antibody CNA1 antibody FLJ26613 antibody MGC52248 antibody PP2BA_HUMAN antibody PPP2B antibody PPP3CA antibody Protein phosphatase 2B catalytic subunit antibody Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody Protein phosphatase 3 catalytic subunit alpha isozyme antibody Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody

Uniprot ID :

Q08209

more info or order :

Boster Immunoleader product webpage