Boster Immunoleader

antibody

Anti-HLA-C Antibody

PB9377

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-HLA-C Antibody

PB9377

Polyclonal

Rabbit IgG polyclonal antibody for MHC class I antigen(HLA-C) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for HLA-C is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C(38-62aa EYWDRETQKYKRQAQADRVNLRKLR).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

1. Ríos A, Rodríguez JM, Moya MR, Galindo PJ, Canteras M, Alvarez MR, Parrilla P (2006). "Associations of HLA-C alleles with multinodular goiters: study in a population from southeastern Spain.". Arch Surg 141 (2): 123–8. 2. Valés-Gómez M, Reyburn HT, Mandelboim M, Strominger JL (September 1998). "Kinetics of interaction of HLA-C ligands with natural killer cell inhibitory receptors". Immunity 9 (3): 337–44.

HLA-C

MHC class I antigen

major histocompatibility complex, class I, C

1C07_HUMAN antibody Cw-7 alpha chain antibody D6S204 antibody HLA class I histocompatibility antigen antibody HLA class I histocompatibility antigen, C alpha chain antibody HLA class I histocompatibility antigen, Cw-1 alpha chain antibody HLA-C antibodyHLA-C histocompatibility type antibody HLA-Cw antibody HLA-JY3 antibody HLAC antibody HLC-C antibody human leukocyte antigen-C alpha chain antibody major histocompatibility antigen HLA-C antibody major histocompatibility complex, class I, C antibody MHC class I antigen antibody MHC class I antigen Cw 1 antibody MHC class I antigen Cw*7 antibody MHC class I antigen heavy chain HLA-C antibody PSORS1 antibody

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