Anti-FRZB Antibody | Boster Immunoleader PB9372 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-FRZB Antibody

catalog :

PB9372

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, ELISA

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-FRZB Antibody

Catalog Number :

PB9372

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 3(FRZB) detection. Tested with WB, ELISA in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,ELISA

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

ELISA :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for FRZB is approximately 0.1ng/lane under reducing conditions. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Reference :

1. Baker-Lepain JC, et al. Variant alleles of the Wnt antagonist FRZB are determinants of hip shape and modify the relationship between hip shape and osteoarthritis. Arthritis Rheum, 2012 May. 2. Qin S, et al. FRZB knockdown upregulates β-catenin activity and enhances cell aggressiveness in gastric cancer. Oncol Rep, 2014 May. 3. Wasmann RA, et al. Novel membrane frizzled-related protein gene mutation as cause of posterior microphthalmia resulting in high hyperopia with macular folds. Acta Ophthalmol, 2014 May.

Gene Name :

FRZB

Protein Name :

Secreted frizzled-related protein 3

Gene Full Name :

frizzled-related protein

Synonyms :

FIZ antibody FRE antibody Frezzled antibody Fritz antibody Frizzled-related protein 1 antibody FRP antibody FrzB 1 antibody FRZB antibody FRZB1 antibody OTTMUSP00000014350 antibody RP23-33L19.1 antibody Secreted frizzled related protein 3 [Precursor] antibody Secreted frizzled related sequence protein 3 antibody sFRP 3 antibody SFRP antibody

Uniprot ID :

Q92765

more info or order :

Boster Immunoleader product webpage