Anti-MASPIN Antibody | Boster Immunoleader PB9356 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MASPIN Antibody

catalog :

PB9356

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Availability :

info changed 20150318

Product Name :

Anti-MASPIN Antibody

Catalog Number :

PB9356

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Serpin B5(SERPINB5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for MASPIN is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Reference :

1. Blandamura S, et al. Maspin expression, subcellular localization and clinicopathological correlation in endometrial hyperplasia and endometrial adenocarcinoma. Histol Histopathol, 2014 Jun. 2. Taskiran C, et al. Maspin expression in endometrial hyperplasia and carcinoma, and its relation with angiogenesis. Eur J Gynaecol Oncol, 2014. 3. Triulzi T, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer, 2014 Jun 15.

Gene Name :

SERPINB5

Protein Name :

Serpin B5

Gene Full Name :

serpin peptidase inhibitor, clade B (ovalbumin), member 5

Synonyms :

mammary serine proteinase inhibitor antibody Maspin antibody Ovalbumin antibody Peptidase inhibitor 5 antibody PI-5 antibody PI5 antibody Protease inhibitor 5 antibody Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody Serpin B5 antibody serpin peptidase inhibitor antibody Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody SERPINB5 antibody SPB5_HUMAN antibody Spi5 antibody Spi7 antibody

Uniprot ID :

P36952

more info or order :

Boster Immunoleader product webpage