Boster Immunoleader

antibody

Anti-Patched/PTCH1 Antibody

PB9352

100 ug/vial

240 USD

polyclonal

rabbit

Anti-Patched/PTCH1 Antibody

PB9352

Polyclonal

Rabbit IgG polyclonal antibody for Protein patched homolog 1(PTCH1) detection. Tested with WB in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat Predicted to work with: human

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

WB: The detection limit for Patched is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Patched recombinant protein (Position: D1219-N1447). Human Patched shares 91% amino acid (aa) sequence identity with mouse Patched.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

PTCH1 is also known as PTC, BCNS or HPE7. This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

1. A loss-of-function mutation in PTCH1 suggests a role for autocrine hedgehog signaling in colorectal tumorigenesis.Chung JH, et al. Oncotarget, 2013 Dec. 2. Basal cell carcinosarcoma with PTCH1 mutations in both epithelial and sarcomatoid primary tumor components and in the sarcomatoid metastasis.Kiuru M, et al. Am J Surg Pathol, 2014 Jan. 3. Proteomic analysis of PTCH1+/- fibroblast lysate and conditioned culture media isolated from the skin of healthy subjects and nevoid basal cell carcinoma syndrome patients.Ponti G, et al. Biomed Res Int, 2013.

PTCH1

Protein patched homolog 1

patched 1

A230106A15Rik antibody BCNS antibody FLJ26746 antibody FLJ42602 antibody Holoprosencephaly 7 antibody HPE7 antibody mes antibody NBCCS antibody OTTHUMP00000021709 antibody OTTHUMP00000021710 antibody Patched antibody Patched (Drosophila) homolog antibody Patched 1 antibody Patched homolog (Drosophila) antibody Patched homolog 1 (Drosophila) antibody Patched homolog 1 antibody Patched protein homolog 1 antibody Protein patched homolog 1 antibody PTC antibody PTC1 antibody PTC1_HUMAN antibody PTCH antibody PTCH protein +12b antibody PTCH protein +4' antibody PTCH protein -10 antibody PTCH protein antibody PTCH1 antibody PTCH1 protein antibody PTCH11 antibody Ptch2 antibody

Q13635