Anti-Peroxiredoxin 1 Antibody | Boster Immunoleader PB9348 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Peroxiredoxin 1 Antibody

catalog :

PB9348

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Availability :

info changed 20150318

Product Name :

Anti-Peroxiredoxin 1 Antibody

Catalog Number :

PB9348

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Peroxiredoxin-1(PRDX1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Peroxiredoxin 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1 (100-128aa MNIPLVSDPKRTIAQDYGVLKADEGISFR), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.

Reference :

1. Neumann, C. A., Krause, D. S., Carman, C. V., Das, S., Dubey, D. P., Abraham, J. L., Bronson, R. T., Fujiwara, Y., Orkin, S. H., Van Etten, R. A.Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature 424: 561-565, 2003. 2. Prosperi, M.-T., Apiou, F., Dutrillaux, B., Goubin, G. Organization and chromosomal assignment of two human PAG gene loci: PAGA encoding a functional gene and PAGB a processed pseudogene. Genomics 19: 236-241, 1994. 3. Yan, Y., Sabharwal, P., Rao, M., Sockanathan, S. The antioxidant enzyme Prdx1 controls neuronal differentiation by thiol-redox-dependent activation of GDE2. Cell 138: 1209-1221, 2009.

Gene Name :

PRDX1

Protein Name :

Peroxiredoxin-1

Gene Full Name :

peroxiredoxin 1

Synonyms :

Hbp23 antibody Heme binding 23 kDa protein antibody Macrophage 23 Kd stress protein antibody Macrophage 23kDa stress protein antibody Macrophase stress protein 22kDa antibody Macrophase stress protein 23 kd antibody MSP23 antibody Natural killer cell enhancing factor A antibody Natural killer cell-enhancing factor A antibody Natural killer enhancing factor A antibody NKEF A antibody NKEF-A antibody NkefA antibody OSF3 antibody Osteoblast specific factor 3 antibody PAG antibody Paga antibody PAGB antibody Peroxiredoxin-1 antibody Prdx 1 antibody Prdx1 antibody PRDX1_HUMAN antibody Proliferation associated gene A antibody Proliferation associated protein PAG antibody Proliferation-associated gene protein antibody PrxI antibody Tdpx 2 antibody Tdpx2 antibody TDX2 antibody Thioredoxin dependent peroxide reductase 2 antibody Thioredoxin peroxidase 2 antibody Thioredoxin-dependent peroxide reductase 2 antibody TPxA antibody Trx dependent peroxide reductase 2 antibody

Uniprot ID :

Q06830

more info or order :

Boster Immunoleader product webpage