Anti-Caspase-8 Antibody | Boster Immunoleader PB9331 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Caspase-8 Antibody

catalog :

PB9331

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Caspase-8 Antibody

Catalog Number :

PB9331

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Caspase-8(CASP8) detection. Tested with WB in Mouse.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Notes :

WB: The detection limit for Caspase-8 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived mouse Caspase-8 recombinant protein (Position: S388-P480). Mouse Caspase-8 shares 83% amino acid (aa) sequence identity with human Caspase-8.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Reference :

1. Activated neutrophils induce epithelial cell apoptosis through oxidant-dependent tyrosine dephosphorylation of caspase-8.Jia SH, et al. Am J Pathol, 2014 Apr. 2. Intra- and interdimeric caspase-8 self-cleavage controls strength and timing of CD95-induced apoptosis.Kallenberger SM, et al. Sci Signal, 2014 Mar 11. 3. Association between CASP8 -652 6N del polymorphism (rs3834129) and colorectal cancer risk: results from a multi-centric study.Pardini B, et al. PLoS One, 2014.

Gene Name :

CASP8

Protein Name :

Caspase-8

Gene Full Name :

caspase 8, apoptosis-related cysteine peptidase

Synonyms :

ALPS2B antibody Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody Apoptosis related cysteine peptidase antibody Apoptotic cysteine protease antibody Apoptotic protease Mch 5 antibody Apoptotic protease Mch-5 antibody Apoptotic protease Mch5 antibody CAP 4 antibody CAP4 antibody CASP 8 antibody CASP-8 antibody CASP8 antibody CASP8_HUMAN antibody Caspase 8 antibody Caspase 8 apoptosis related cysteine peptidase antibody Caspase-8 subunit p10 antibody Caspase8 antibody CED 3 antibody FADD homologous ICE/CED 3 like protease antibody FADD Homologous ICE/CED3 Like Protease antibody FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease antibody FADD-like ICE antibody FLICE antibody FLJ17672 antibody ICE like apoptotic protease 5 antibody ICE-like apoptotic protease 5 antibody MACH alpha 1/2/3 protein antibody MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5 antibody MCH5 antibody MGC78473 antibody MORT1 associated CED 3 homolog antibody MORT1 associated CED3 homolog antibody MORT1-associated CED-3 homolog antibody OTTHUMP00000163717 antibody OTTHUMP00000163720 antibody OTTHUMP00000163724 antibody OTTHUMP00000163725 antibody OTTHUMP00000165062 antibody OTTHUMP00000165063 antibody OTTHUMP00000165064 antibody OTTHUMP00000206552 antibody OTTHUMP00000206582 antibody

Uniprot ID :

O89110

more info or order :

Boster Immunoleader product webpage