Boster Immunoleader

antibody

Anti-Calpain 1 Antibody

PB9330

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Calpain 1 Antibody

PB9330

Polyclonal

Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for Calpain 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar. 2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012.

CAPN1

Calpain-1 catalytic subunit

calpain 1, (mu/I) large subunit

Ca2 activated neutral protease antibody Calcium activated neutral proteinase 1 antibody Calcium activated neutral proteinase antibody Calcium activated neutral proteinase small subunit antibody Calcium dependent protease small subunit 1 antibody Calcium dependent protease small subunit antibody Calcium-activated neutral proteinase 1 antibody Calpain 1 (mu/I) large subunit antibody Calpain 1 antibody Calpain 1 catalytic subunit antibody Calpain 1 large subunit antibody Calpain 1, (mu/I) large subunit antibody Calpain Large Polypeptide L1 antibody Calpain mu type antibody Calpain mu-type antibody Calpain regulatory subunit antibody Calpain small subunit 1 antibody Calpain, large polypeptide L1 antibody Calpain-1 catalytic subunit antibody Calpain-1 large subunit antibody Calpain1 antibody CAN1_HUMAN antibody CANP 1 antibody CANP antibody CANP small subunit antibody CANP1 antibody CANPL 1 antibody CANPL1 antibody CAPN 1 antibody CAPN1 antibody Cell proliferation inducing protein 30 antibody Cell proliferation-inducing gene 30 protein antibody Micromolar Calpain antibody Micromolar-calpain antibody Mu Calpain antibody muCANP antibody muCL antibody

P07384