Anti-Hsp47 Antibody | Boster Immunoleader PB9325 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hsp47 Antibody

catalog :

PB9325

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Hsp47 Antibody

Catalog Number :

PB9325

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Serpin H1(SERPINH1) detection. Tested with WB, IHC-P in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Notes :

WB: The detection limit for Hsp47 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.

Reference :

1. Christiansen, H. E., Schwarze, U., Pyott, S. M., AlSwaid, A., Al Balwi, M., Alrasheed, S., Pepin, M. G., Weis, M. A., Eyre, D. R., Byers, P. H. Homozygosity for a missense mutation in SERPINH1, which encodes the collagen chaperone protein HSP47, results in severe recessive osteogenesis imperfecta. Am. J. Hum. Genet. 86: 389-398, 2010. 2. "Entrez Gene: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)" 3. Dafforn T, Della M, Miller A (2001). "The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis". J. Biol. Chem. 276(52): 49310–9.

Gene Name :

SERPINH1

Protein Name :

Serpin H1

Gene Full Name :

serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

Synonyms :

47 kDa heat shock protein antibody 47 kDa heat shock protein precursor antibody Arsenic transactivated protein 3 antibody Arsenic-transactivated protein 3 antibody AsTP 3 antibody AsTP3 antibody CBP 1 antibody CBP 2 antibody CBP1 antibody CBP2 antibody Cell proliferation-inducing gene 14 protein antibody Collagen binding protein 1 antibody Collagen binding protein 2 antibody Collagen binding protein antibody Collagen-binding protein 2 antibody Collagen-binding protein antibody Colligen antibody Colligin 1 antibody Colligin 2 antibody Colligin antibody colligin-1 antibody colligin-2 antibody gp46 antibody Heat shock protein 47 antibody Heat-shock protein 47 antibody HGNC 1547 antibody Hsp 47 antibody J6 antibody OI10 antibody PIG 14 antibody PIG14 antibody PPROM antibody Proliferation inducing gene 14 antibody Proliferation inducing gene 14 protein antibody RA A47 antibody RA-A47 antibody Rheumatoid arthritis antigen A 47 antibody rheumatoid arthritis antigen A-47 antibody Rheumatoid arthritis related antigen RA A47 antibody Rheumatoid arthritis-related antigen RA-A47 antibody serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody Serine or cysteine proteinase inhibitor clade H member 1 antibody Serine or cysteine proteinase inhibitor clade H member 2 antibody SERPH_HUMAN antibody Serpin H1 antibody Serpin peptidase inhibitor clade H member 1 antibody serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody Serpin peptidase inhibitor, clade H, member 1 antibody SERPINH1 antibody SERPINH2 antibody

Uniprot ID :

P50454

more info or order :

Boster Immunoleader product webpage