Anti-NF-kB p65 Antibody | Boster Immunoleader PB9324 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-NF-kB p65 Antibody

catalog :

PB9324

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot

publications citing this reagent :

Liu M, Wang Y, Qian C, Li H. Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis. Int J Mol Med. 2014;34:1492-504 pubmed publisher
Sun C, Lin H, Yu W, Li X, Chen Y, Qiu X, et al. Neurotrophic effect of bone marrow mesenchymal stem cells for erectile dysfunction in diabetic rats. Int J Androl. 2012;35:601-7 pubmed publisher
Yan Q, Ruan J, Ding Y, Li W, Li Y, Zeng Y. Electro-acupuncture promotes differentiation of mesenchymal stem cells, regeneration of nerve fibers and partial functional recovery after spinal cord injury. Exp Toxicol Pathol. 2011;63:151-6 pubmed publisher

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Boster Immunoleader product webpage

product information

Product Name :

Anti-NF-kB p65 Antibody

Catalog Number :

PB9324

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat

Notes :

WB: The detection limit for NF-kB p65 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived mouse NF-kB p65 recombinant protein (Position: D291-Q479). Mouse NF-kB p65 shares 77% amino acid (aa) sequence identity with human NF-kB p65.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein encoded by the RELA gene. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

Reference :

1. Nolan GP, Ghosh S, Liou HC, Tempst P, Baltimore D (April 1991). "DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappa B, a rel-related polypeptide". Cell 64 (5): 961–9. 2. Lim, C.-A., Yao, F., Wong, J. J.-Y., George, J., Xu, H., Chiu, K. P., Sung, W.-K., Lipovich, L., Vega, V. B., Chen, J., Shahab, A., Zhao, X. D., Hibberd, M., Wei, C.-L., Lim, B., Ng, H.-H., Ruan, Y., Chin, K.-C. Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappa-B upon TLR4 activation.Molec. Cell 27: 622-635, 2007. 3. Mazzeo, A., Aguennouz, M., Messina, C., Vita, G. Immunolocalization and activation of transcription factor nuclear factor kappa-B in dysimmune neuropathies and familial amyloidotic polyneuropathy. Arch. Neurol. 61: 1097-1102, 2004.

Gene Name :

RELA

Protein Name :

Transcription factor p65

Gene Full Name :

v-rel avian reticuloendotheliosis viral oncogene homolog A

Synonyms :

Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody MGC131774 antibody NF kappa B p65delta3 antibody NFKB 3 antibody NFKB3 antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear factor NF-kappa-B p65 subunit antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65 (avian) antibody OTTHUMP00000233473 antibody OTTHUMP00000233474 antibody OTTHUMP00000233475 antibody OTTHUMP00000233476 antibody OTTHUMP00000233900 antibody p65 antibody p65 NF kappaB antibody p65 NFkB antibody RELA antibody TF65_HUMAN antibodyTranscription Factor p65 antibody v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody v rel reticuloendotheliosis viral oncogene homolog A antibody V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody V-rel avian reticuloendotheliosis viral oncogene homolog A antibody

Uniprot ID :

Q04207

more info or order :

Boster Immunoleader product webpage