Boster Immunoleader

antibody

Anti-Rad51 Antibody

PB9323

100 ug/vial

240 USD

polyclonal

rabbit

human, rat

western blot, immunohistochemistry - paraffin section

Anti-Rad51 Antibody

PB9323

Polyclonal

Rabbit IgG polyclonal antibody for DNA repair protein RAD51 homolog 1(RAD51) detection. Tested with WB, IHC-P in Human;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

WB: The detection limit for Rad51 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

1. Kato, M.; Yano, K.; Matsuo, F.; Saito, H.; Katagiri, T.; Kurumizaka, H.; Yoshimoto, M.; Kasumi, F.; Akiyama, F.; Sakamoto, G.; Nagawa, H.; Nakamura, Y.; Miki, Y. : Identification of Rad51 alteration in patients with bilateral breast cancer. J. Hum. Genet. 45: 133-137, 2000. 2. Daniel DC (2002). "Highlight: BRCA1 and BRCA2 proteins in breast cancer.". Microsc. Res. Tech. 59 (1): 68–83.

RAD51

DNA repair protein RAD51 homolog 1

RAD51 recombinase

BRCA1/BRCA2 containing complex, subunit 5 antibody BRCC 5 antibody BRCC5 antibody DNA repair protein RAD51 homolog 1 antibody DNA repair protein rhp51 antibody E coli RecA homolog antibody Homolog of E coli RecA antibody homolog of S cerevisiae RAD51 antibody HRAD51 antibody HsRad51 antibody HsT16930 antibody MRMV2 antibody Rad 51 antibody RAD51 antibody RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody RAD51 homolog A antibody RAD51 homolog antibody RAD51 homolog S. cerevisiae antibody RAD51 recombinase antibody RAD51 S cerevisiae homolog antibody RAD51, S. cerevisiae, homolog of antibody RAD51_HUMAN antibody RAD51A antibody RECA antibody RecA homolog E. coli antibody RecA like protein antibody RecA, E. coli, homolog of antibody recombination protein A antibody

Q06609