Anti-Rab3A Antibody | Boster Immunoleader PB9322 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Rab3A Antibody

catalog :

PB9322

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Rab3A Antibody

Catalog Number :

PB9322

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Ras-related protein Rab-3A(RAB3A) detection. Tested with WB in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Notes :

WB: The detection limit for Rab3A is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A (192-220aa DTADPAVTGAKQGPQLSDQQVPPHQDCAC), different from the related mouse and rat sequences by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. This gene is mapped to 19p13.11. Synapsin I stimulated the RAB3A cycle by increasing GTP binding, GTPase activity, and RAB3A recruitment to the synaptic vesicle membrane. Conversely, RAB3A inhibited synapsin I binding to actin and synapsin I-induced synaptic vesicle clustering. It has been found that RAB3A was necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, RAB3A has been found to regulate a late step in synaptic vesicle fusion.

Reference :

1. Giovedi, S., Darchen, F., Valtorta, F., Greengard, P., Benfenati, F. Synapsin is a novel Rab3 effector protein on small synaptic vesicles. II. Functional effects of the Rab3A-synapsin I interaction. J. Biol. Chem. 279: 43769-43779, 2004. 2. Huang, Y.-Y., Zakharenko, S. S., Schoch, S., Kaeser, P. S., Janz, R., Sudhof, T. C., Siegelbaum, S. A., Kandel, E. R. Genetic evidence for a protein-kinase-A-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic potentiation. Proc. Nat. Acad. Sci. 102: 9365-9370, 2005. 3. Rousseau-Merck M, Zahraoui A, Bernheim A, Touchot N, Miglierina R, Tavitian A et al. (January 1990). "Chromosome mapping of the human ras-related rab3A gene to 19p13.2". Genomics 5 (4): 694–8.

Gene Name :

RAB3A

Protein Name :

Ras-related protein Rab-3A

Gene Full Name :

RAB3A, member RAS oncogene family

Synonyms :

Rab 3A antibody RAB 3A member RAS oncogene family antibody Rab3a antibody RAB3A member RAS oncogene family antibody RAB3A_HUMAN antibody RAS associated protein RAB 3A antibody RAS associated protein RAB3A antibody Ras related protein Rab 3A antibody Ras related protein Rab3A antibody Ras-related protein Rab-3A antibody

Uniprot ID :

P20336

more info or order :

Boster Immunoleader product webpage