Boster Immunoleader

antibody

Anti-PKC epsilon Antibody

PB9320

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-PKC epsilon Antibody

PB9320

Polyclonal

Rabbit IgG polyclonal antibody for Protein kinase C epsilon type(PRKCE) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

WB: The detection limit for PKC epsilon is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.

1. Basta P, Strickland MB, Holmes W, Loomis CR, Ballas LM, Burns DJ (Nov 1992). "Sequence and expression of human protein kinase C-epsilon". Biochim Biophys Acta 1132 (2): 154–60. 2. Knauf, J. A., Elisei, R., Mochly-Rosen, D., Liron, T., Chen, X.-N., Gonsky, R., Korenberg, J. R., Fagin, J. A. Involvement of protein kinase C-epsilon (PKC-epsilon) in thyroid cell death: a truncated chimeric PKC-epsilon cloned from a thyroid cancer cell line protects thyroid cells from apoptosis. J. Biol. Chem. 274: 23414-23425, 1999. 3. Premkumar, L. S., Ahern, G. P. Induction of vanilloid receptor channel activity by protein kinase C. Nature 408: 985-990, 2000.

PRKCE

Protein kinase C epsilon type

protein kinase C, epsilon

KPCE_HUMAN antibody MGC125656 antibody MGC125657 antibody nPKC epsilon antibody nPKC-epsilon antibody PKCE antibody Pkcea antibody PRKCE antibody Protein kinase C epsilon antibody Protein kinase C epsilon type antibody

Q02156