Boster Immunoleader

antibody

Anti-Oct-1 Antibody

PB9317

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Oct-1 Antibody

PB9317

Polyclonal

Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for Oct-1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.

1. Hsieh, C.-L., Sturm, R., Herr, W., Francke, U. The gene for the ubiquitous octamer-binding protein Oct-1 is on human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on mouse chromosome 1. Genomics 6: 666-672, 1990. 2. Tomilin, A., Remenyi, A., Lins, K., Bak, H., Leidel, S., Vriend, G., Wilmanns, M., Scholer, H. R.Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Cell 103: 853-864, 2000. 3. Roberts S, Segil N, Heintz N (October 1991). "Differential phosphorylation of the transcription factor Oct1 during the cell cycle". Science 253 (5023): 1022–6.

POU2F1

POU domain, class 2, transcription factor 1

POU class 2 homeobox 1

FLJ42836 antibody NF A1 antibody NF-A1 antibody Oct 1 antibody Oct 1B antibody Oct-1 antibody OCT1 antibody Octamer binding protein 1 antibody Octamer binding transcription factor 1 antibody Octamer-binding protein 1 antibody Octamer-binding transcription factor 1 antibody OTF 1 antibody OTF-1 antibody OTF1 antibody OTTHUMP00000032348 antibody OTTHUMP00000032350 antibody OTTHUMP00000032351 antibody PO21 antibody PO2F1 antibody PO2F1_HUMAN antibody POU class 2 homeobox 1 antibody POU domain class 2 transcription factor 1 antibody POU domain, class 2, transcription factor 1 antibody POU2F1 antibody

P14859