Anti-PIK3CA Antibody | Boster Immunoleader PB9314 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PIK3CA Antibody

catalog :

PB9314

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PIK3CA Antibody

Catalog Number :

PB9314

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform(PIK3CA) detection. Tested with WB in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Notes :

WB: The detection limit for PIK3CA is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human PIK3CA recombinant protein (Position: H936-N1068). Human PIK3CA shares 98% amino acid (aa) sequence identity with mouse PIK3CA.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Phosphatidylinositol-4,5-bisphosphate 3-kinase, also called PIK3CA, is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. PIK3CA gene is mapped to 3q26.32. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate phosphatidylinositols (PtdIns), PtdIns4P andPtdIns(4,5)P2. Recent evidence has shown that the PIK3CA gene is mutated in a range of human cancers. It has been found to be oncogenic and has been implicated in cervical cancers. PIK3CA mutations in breast cancer may be a predictive marker to guide the selection of patients who would benefit from mTOR inhibitor therapy. In addition to that, the presence of PIK3CA mutation may predict response to aspirin therapy for colorectal cancer, indicating power and promise of "Molecular Pathological Epidemiology (MPE)" approach as well as a complex interaction within the tumor microenvironment in this phenomenon.

Reference :

1. Hiles I, Otsu M, Volinia S, Fry M, Gout I, Dhand R et al. (August 1992). "Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit". Cell 70 (3): 419–29. 2. Ma Y, Wei S, Lin Y, Lung J, Chang T, Whang-Peng J et al. (May 2000). "PIK3CA as an oncogene in cervical cancer". Oncogene 19 (23): 2739–44. 3. Weigelt, B., Warne, P. H., Downward, J. PIK3CA mutation, but not PTEN loss of function, determines the sensitivity of breast cancer cells to mTOR inhibitory drugs. Oncogene 30: 3222-3233, 2011.

Gene Name :

PIK3CA

Protein Name :

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Gene Full Name :

phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

Synonyms :

5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody caPI3K antibody CLOVE antibody CWS5 antibody MCAP antibody MCM antibody MCMTC antibody MGC142161 antibody MGC142163 antibody p110 alpha antibody p110alpha antibody Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit alpha isoform antibody Phosphatidylinositol-4 antibody Phosphoinositide 3 kinase catalytic alpha polypeptide antibody PI3 kinase p110 subunit alpha antibody PI3-kinase subunit alpha antibody PI3K antibody PI3K-alpha antibody PI3KC A antibody PIK3C A antibody Pik3ca antibody PK3CA antibody PK3CA_HUMAN antibody PtdIns 3 kinase p110 antibody PtdIns-3-kinase subunit alpha antibody PtdIns-3-kinase subunit p110-alpha antibody Serine/threonine protein kinase PIK3CA antibody

Uniprot ID :

P42336

more info or order :

Boster Immunoleader product webpage