Boster Immunoleader

antibody

Anti-Parkin Antibody

PB9307

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

info changed 20150318

Anti-Parkin Antibody

PB9307

Polyclonal

Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase parkin(PARK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse

WB: The detection limit for Parkin is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.

1. Huynh, D. P., Scoles, D. R., Ho, T. H., Del Bigio, M. R., Pulst, S.-M. Parkin is associated with actin filaments in neuronal and nonneural cells. Ann. Neurol. 48: 737-744, 2000. 2. Kitada T, Asakawa S, Hattori N, Matsumine H, Yamamura Y, Minoshima S et al. (Apr 1998). "Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism". Nature 392 (6676): 605–608. 3. Rothfuss, O., Fischer, H., Hasegawa, T., Maisel, M., Leitner, P., Miesel, F., Sharma, M., Bornemann, A., Berg, D., Gasser, T., Patenge, N. Parkin promotes mitochondrial genome integrity and supports mitochondrial DNA repair. Hum. Molec. Genet. 18: 3832-3850, 2009.

PARK2

E3 ubiquitin-protein ligase parkin

parkin RBR E3 ubiquitin protein ligase

AR JP antibody E3 ubiquitin ligase antibody E3 ubiquitin protein ligase parkin antibody E3 ubiquitin-protein ligase parkin antibody FRA6E antibody LPRS 2 antibody LPRS2 antibody PARK 2 antibody PARK2 antibody Parkin 2 antibody Parkinson disease (autosomal recessive juvenile) 2 antibody Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody Parkinson disease protein 2 antibody Parkinson juvenile disease protein 2 antibody Parkinson protein 2 E3 ubiquitin protein ligase antibody Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody PDJ antibody PRKN 2 antibody PRKN antibody PRKN2 antibody PRKN2_HUMAN antibody Ubiquitin E3 ligase PRKN antibody

O60260