Boster Immunoleader

antibody

Anti-PAPP A Antibody

PB9306

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-PAPP A Antibody

PB9306

Polyclonal

Rabbit IgG polyclonal antibody for Pappalysin-1(PAPPA) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for PAPP A is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. It is mapped to 9q33.1. PAPPA is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPPA is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 molar ratio.

1. Oxvig, C., Sand, O., Kristensen, T., Gleich, G. J., Sottrup-Jensen, L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. 268: 12243-12246, 1993. 2. Overgaard, M. T., Haaning, J., Boldt, H. B., Olsen, I. M., Laursen, L. S., Christiansen, M., Gleich, G. J., Sottrup-Jensen, L., Conover, C. A., Oxvig, C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J. Biol. Chem. 275: 31128-31133, 2000.

PAPPA

Pappalysin-1

pregnancy-associated plasma protein A, pappalysin 1

ASBABP2 antibody Aspecific BCL2 ARE binding protein 2 antibody Differentially placenta 1 expressed protein antibody DIPLA1 antibody IGF dependent IGFBP 4 protease antibody IGF-dependent IGFBP-4 protease antibody IGFBP 4ase antibody IGFBP-4ase antibody IGFBP4ase antibody Insulin like growth factor dependent IGF binding protein 4 protease antibody Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody PAPA antibody PAPP A antibody PAPP-A antibody PAPP1_HUMAN antibody PAPPA antibody PAPPA1 antibody Pappalysin 1 precursor antibody Pappalysin-1 antibody Pregnancy Associated Plasma Protein A antibody Pregnancy associated plasma protein A pappalysin 1 antibody Pregnancy-associated plasma protein A antibody

Q13219