Boster Immunoleader

antibody

Anti-P2X2 Antibody

PB9303

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-P2X2 Antibody

PB9303

Polyclonal

Rabbit IgG polyclonal antibody for P2X purinoceptor 2(P2RX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for P2X2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

The P2RX2 gene encodes the P2X2 receptor, which assembles as a trimer to form a ligand-gated ion channel gated by extracellular ATP. P2X2 receptors mediate a variety of cellular responses, including excitatory postsynaptic responses in sensory neurons. The product of this gene belongs to the family of purinoceptors for ATP. P2RX2 is mapped to 12q24.33. It has been found that ATP-activated P2RX2 influenced OHC electromotility, a stimulus-induced change in hair cell length that functions as an amplifier to determine hearing sensitivity and frequency selectivity. What’s more, P2RX2 channels were necessary for development of the temporary threshold shift.

1. Housley, G. D., Morton-Jones, R., Vlajkovic, S. M., Telang, R. S., Paramananthasivam, V., Tadros, S. F., Wong, A. C. Y., Froud, K. E., Cederholm, J. M. E., Sivakumaran, Y., Snguanwongchai, P., Khakh, B. S., Cockayne, D. A., Thorne, P. R., Ryan, A. F. ATP-gated ion channels mediate adaptation to elevated sound levels. Proc. Nat. Acad. Sci. 110: 7494-7499, 2013. 2. Yan, D., Zhu, Y., Walsh, T., Xie, D., Yuan, H., Sirmaci, A., Fujikawa, T., Wong, A. C. Y., Loh, T. L., Du, L., Grati, M., Vlajkovic, S. M., and 10 others. Mutation of the ATP-gated P2X(2) receptor leads to progressive hearing loss and increased susceptibility to noise. Proc. Nat. Acad. Sci. 110: 2228-2233, 2013. 3. Yu, N., Zhao, H.-B. ATP activates P2x receptors and requires extracellular Ca(++) participation to modify outer hair cell nonlinear capacitance. Pflugers Arch. 457: 453-461, 2008.

P2RX2

P2X purinoceptor 2

purinergic receptor P2X, ligand gated ion channel, 2

ATP receptor antibody MGC129601 antibody P2RX 2 antibody P2RX2 antibody P2RX2_HUMAN antibody P2X purinoceptor 2 antibody P2X Receptor subunit 2 antibody P2X2 antibody P2X2a antibody Purinergic receptor antibody Purinergic receptor P2X ligand gated ion channel 2 antibody Purinergic receptor P2X2 isoform A antibody Purinergic receptor P2X2 isoform B antibody Purinergic receptor P2X2 isoform C antibody Purinergic receptor P2X2 isoform D antibody Purinergic receptor P2X2 isoform H antibody Purinergic receptor P2X2 isoform I antibody

Q9UBL9