Boster Immunoleader

antibody

Anti-NUP98 Antibody

PB9302

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

Anti-NUP98 Antibody

PB9302

Polyclonal

Rabbit IgG polyclonal antibody for Nuclear pore complex protein Nup98-Nup96(NUP98) detection. Tested with WB in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for NUP98 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human NUP98 recombinant protein (Position: H549-F880). Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene. This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC. NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis. It also plays roles in gene expression, mitotic checkpoint, and pathogenesis. Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates. In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.

1. Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W., Kutay, U. Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell 144: 539-550, 2011. 2. Nakamura, T., Largaespada, D. A., Lee, M. P., Johnson, L. A., Ohyashiki, K., Toyama, K., Chen, S. J., Willman, C. L., Chen, I.-M., Feinberg, A. P., Jenkins, N. A., Copeland, N. G., Shaughnessy, J. D., Jr. Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia. Nature Genet. 12: 154-158, 1996. 3. von Kobbe, C., van Deursen, J. M. A., Rodrigues, J. P., Sitterlin, D., Bachi, A., Wu, X., Wilm, M., Carmo-Fonseca, M., Izaurralde, E. Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98. Molec. Cell 6: 1243-1252, 2000.

NUP98

Nuclear pore complex protein Nup98-Nup96

nucleoporin 98kDa

96 kDa nucleoporin antibody 98 kDa nucleoporin antibody ADAR2 antibody ADIR2 antibody GLFG-repeat containing nucleoporin antibody Nuclear pore complex protein Nup96 antibody Nuclear pore complex protein Nup98 Nup96 antibody Nucleoporin 98kD antibody nucleoporin 98kDa antibody Nucleoporin Nup96 antibody Nucleoporin Nup98 antibody NUP196 antibody NUP96 antibody Nup98 antibody Nup98-Nup96 antibody NUP98_HUMAN antibody

P52948