Boster Immunoleader

antibody

Anti-CARD4 Antibody

PB9294

100 ug/vial

240 USD

polyclonal

rabbit

human, rat

western blot, immunohistochemistry - paraffin section

Anti-CARD4 Antibody

PB9294

Polyclonal

Rabbit IgG polyclonal antibody for Nucleotide-binding oligomerization domain-containing protein 1(NOD1) detection. Tested with WB, IHC-P in Human;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for CARD4 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD). This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.

1. Chamaillard, M., Hashimoto, M., Horie, Y., Masumoto, J., Su, Q., Saab, L., Ogura, Y., Kawasaki, A., Fukase, K., Kusumoto, S., Valvano, M. A., Foster, S. J., Mak, T. W., Nunez, G., Inohara, N. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nature Immun. 4: 702-707, 2003. 2. da Silva Correia, J., Miranda, Y., Austin-Brown, N., Hsu, J., Mathison, J., Xiang, R., Zhou, H., Li, Q., Han, J., Ulevitch, R. J. Nod1-dependent control of tumor growth. Proc. Nat. Acad. Sci. 103: 1840-1845, 2006. 3. Martinon F, Tschopp J (2006). "NLRs join TLRs as innate sensors of pathogens.".Trends Immunol. 26 (8): 447–54.

NOD1

Nucleotide-binding oligomerization domain-containing protein 1

nucleotide-binding oligomerization domain containing 1

CARD 4 antibody CARD4 antibody Caspase recruitment domain 4 antibody Caspase recruitment domain containing protein 4 antibody Caspase recruitment domain family member 4 antibody Caspase recruitment domain protein 4 antibody Caspase recruitment domain-containing protein 4 antibody CLR 7.1 antibody CLR7.1 antibody NLR family CARD domain containing 1 antibody NLRC 1 antibody NLRC1 antibody NOD 1 antibody Nod1 antibody NOD1 protein antibody NOD1_HUMAN antibody Nucleotide binding oligomerization domain containing 1 antibody Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody Nucleotide-binding oligomerization domain-containing protein 1 antibody Protein Nod1 antibody

Q9Y239