Anti-IKB beta Antibody | Boster Immunoleader PB9292 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-IKB beta Antibody

catalog :

PB9292

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-IKB beta Antibody

Catalog Number :

PB9292

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for NF-kappa-B inhibitor beta(NFKBIB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for IKB beta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Reference :

1. Okamoto T, Ono T, Hori M, Yang J, Tetsuka T, Kawabe T et al. (November 1998). "Assignment of the IkappaB-beta gene NFKBIB to human chromosome band 19q13.1 by in situ hybridization". Cytogenet Cell Genet 82 (1–2): 105–6. 2. Rao, P., Hayden, M. S., Long, M., Scott, M. L., West, A. P., Zhang, D., Oeckinghaus, A., Lynch, C., Hoffmann, A., Baltimore, D., Ghosh, S. I-kappa-B-beta acts to inhibit and activate gene expression during the inflammatory response. Nature 466: 1115-1119, 2010.

Gene Name :

NFKBIB

Protein Name :

NF-kappa-B inhibitor beta

Gene Full Name :

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta

Synonyms :

I kappa B beta antibody I-kappa-B-beta antibody IkappaBbeta antibody IKB beta antibody IkB-B antibody IkB-beta antibody IKBB antibody IKBB_HUMAN antibody IkBbeta antibody NF kappa BIB antibody NF-kappa-B inhibitor beta antibody NF-kappa-BIB antibody Nfkbib antibody Thyroid receptor interacting protein 9 antibody Thyroid receptor-interacting protein 9 antibody TR interacting protein 9 antibody TR-interacting protein 9 antibody TRIP-9 antibody TRIP9 antibody

Uniprot ID :

Q15653

more info or order :

Boster Immunoleader product webpage