Boster Immunoleader

antibody

Anti-Nrf2 Antibody

PB9290

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Nrf2 Antibody

PB9290

Polyclonal

Rabbit IgG polyclonal antibody for Nuclear factor erythroid 2-related factor 2(NFE2L2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for Nrf2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Nrf2 recombinant protein (Position: Q14-D178). Human Nrf2 shares 79% and 82% amino acid (aa) sequence identity with mouse and rat Nrf2, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

NFE2L2(nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-directed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas(G12D) and BRaf(V619E), and in human pancreatic cancer.

1. Chan, J. Y., Cheung, M.-C., Moi, P., Chan, K., Kan, Y. W. Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization. Hum. Genet. 95: 265-269, 1995. 2. He, C. H., Gong, P., Hu, B., Stewart, D., Choi, M. E., Choi, A. M. K., Alam, J. Identification of activating transcription factor 4 (ATF4) as an Nrf2-interacting protein: implication for heme oxygenase-1 gene regulation. J. Biol. Chem. 276: 20858-20865, 2001. 3. Lee, J.-M., Chan, K., Kan, Y. W., Johnson, J. A. Targeted disruption of Nrf2 causes regenerative immune-mediated hemolytic anemia.Proc. Nat. Acad. Sci. 101: 9751-9756, 2004.

NFE2L2

Nuclear factor erythroid 2-related factor 2

nuclear factor, erythroid 2-like 2

erythroid derived 2 antibody HEBP1 antibody NF E2 related factor 2 antibody NF-E2-related factor 2 antibody NF2L2_HUMAN antibody NFE2 related factor 2 antibody NFE2-related factor 2 antibody NFE2L2 antibody Nrf 2 antibody NRF2 antibody Nuclear factor antibody nuclear factor erythroid 2 like 2 antibody Nuclear factor erythroid 2 related factor 2 antibody Nuclear factor erythroid 2-related factor 2 antibody Nuclear factor erythroid derived 2 like 2 antibody

Q16236