Anti-MSH3 Antibody | Boster Immunoleader PB9285 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MSH3 Antibody

catalog :

PB9285

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MSH3 Antibody

Catalog Number :

PB9285

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA mismatch repair protein Msh3(MSH3) detection. Tested with WB in Human;Mouse.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse

Notes :

WB: The detection limit for MSH3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human MSH3 recombinant protein (Position: E434-N739). Human MSH3 shares 83% amino acid (aa) sequence identity with mouse MSH3.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

DNA mismatch repair protein, MutS Homolog 3 (MSH3), also known as MRP1 or DUP, is a human homologue of the bacterial mismatch repair protein MutS that participates in the mismatch repair (MMR) system. It is mapped to 5q14.1. The primary function of MSH3 is to maintain the stability of the genome and enact tumor suppression by forming the heterodimer MutSβ to correct long insertion/deletion loops and base-base mispairs. The most significant role of MSH3 in cancer is the suppression of tumors by repair of somatic mutations in DNA that occur as the result of base-base mispairs and insertion/deletion loops. Both loss of expression and over expression of MSH3 can leader tocarcinogenic effects. What’s more, it has been shown that inactivation of the MSH3 gene may be involved in the development of hematologic malignancies.

Reference :

1. Gao, Jian-Xin; Park, Jae Myung; Huang, Shengbing; Tougeron, David; Sinicrope, Frank A. (2013). "MSH3 Mismatch Repair Protein Regulates Sensitivity to Cytotoxic Drugs and a Histone Deacetylase Inhibitor in Human Colon Carcinoma Cells". PLoS ONE 8 (5): e65369. 2. Gupta S, Gellert M, Yang W (2012). "Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops.". Nat Struct Mol Biol 19 (1): 72–8. 3. Inokuchi, K., Ikejima, M., Watanabe, A., Nakajima, E., Orimo, H., Nomura, T., Shimada, T. Loss of expression of the human MSH3 gene in hematological malignancies. Biochem. Biophys. Res. Commun. 214: 171-179, 1995.

Gene Name :

MSH3

Protein Name :

DNA mismatch repair protein Msh3

Gene Full Name :

mutS homolog 3

Synonyms :

Divergent upstream protein antibody DNA mismatch repair protein antibody DNA mismatch repair protein Msh 3 antibodyDNA mismatch repair protein Msh3 antibody DUC 1 antibody DUC1 antibody DUG antibody DUP antibody hMSH3 antibody MGC163306 antibody MGC163308 antibody Mismatch repair protein 1 antibody MRP 1 antibody MRP1 antibody MSH 3 antibody MSH3 antibody MSH3_HUMAN antibody MutS homolog 3 (E. coli) antibody MutS homolog 3 antibody

Uniprot ID :

P20585

more info or order :

Boster Immunoleader product webpage