Anti-Mad2L1 Antibody | Boster Immunoleader PB9282 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Mad2L1 Antibody

catalog :

PB9282

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Mad2L1 Antibody

Catalog Number :

PB9282

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD2A(MAD2L1) detection. Tested with WB in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Mad2L1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.

Reference :

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C.Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. "Entrez Gene: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)

Gene Name :

MAD2L1

Protein Name :

Mitotic spindle assembly checkpoint protein MAD2A

Gene Full Name :

MAD2 mitotic arrest deficient-like 1 (yeast)

Synonyms :

Homolog of MAD2 S cerevisiae antibody HsMAD 2 antibody HsMAD2 antibody Human mitotic feedback control protein Madp2 antibody MAD 2 antibody MAD 2A antibody MAD2 antibody MAD2 like 1 antibody MAD2 like protein 1 antibody MAD2 mitotic arrest deficient like 1 antibody MAD2-like protein 1 antibody MAD2A antibody Mad2l1 antibody MD2L1_HUMAN antibody Mitotic arrest deficient 2-like protein 1 antibody Mitotic arrest deficient like 1 antibody Mitotic arrest deficient yeast homolog antibody Mitotic arrest deficient yeast homolog like 1 antibody Mitotic spindle assembly checkpoint protein antibody Mitotic spindle assembly checkpoint protein MAD2A antibody

Uniprot ID :

Q13257

more info or order :

Boster Immunoleader product webpage