Anti-Lamin A/C Antibody | Boster Immunoleader PB9280 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Lamin A/C Antibody

catalog :

PB9280

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Availability :

info changed 20150505

Product Name :

Anti-Lamin A/C Antibody

Catalog Number :

PB9280

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Prelamin-A/C(LMNA) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Lamin A/C is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Lamin A/C recombinant protein (Position: Y481-Y646). Human Lamin A/C shares 90% and 92% amino acid (aa) sequence identity with mouse and rat Lamin A/C, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Reference :

1. Lammerding, J.; Schulze, P. C.; Takahashi, T.; Kozlov, S.; Sullivan, T.; Kamm, R. D.; Stewart, C. L.; Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004. 2. Lin, F.; Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993. 3. Wydner, K. L.; McNeil, J. A.; Lin, F.; Worman, H. J.; Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996.

Gene Name :

LMNA

Protein Name :

Prelamin-A/C

Gene Full Name :

lamin A/C

Synonyms :

70 kDa lamin antibody CDCD1 antibody CDDC antibody CMD1A antibody CMT2B1 antibody EMD2 antibody FPL antibody FPLD antibody HGPS antibody IDC antibody LAMIN A antibody lamin A/C antibody LAMIN C antibody Lamin-A/C antibody LDP1 antibody LFP antibody LGMD1B antibody LMN 1 antibody LMN A antibody LMN C antibody LMN1 antibody LMNA antibody LMNA_HUMAN antibody LMNC antibody NY REN 32 antigen antibody PRO1 antibody Renal carcinoma antigen NY-REN-32 antibody

Uniprot ID :

P02545

more info or order :

Boster Immunoleader product webpage