Boster Immunoleader

antibody

Anti-MSK1 Antibody

PB9268

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MSK1 Antibody

PB9268

Polyclonal

Rabbit IgG polyclonal antibody for Ribosomal protein S6 kinase alpha-5(RPS6KA5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for MSK1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded by the RPS6KA5 gene. It belongs to the AGC family of kinases. MSK1 gene is mapped to chromosome 14q31-q32. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. It has been found that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.

1. Deak, M., Clifton, A. D., Lucocq, J. M., Alessi, D. R. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998. 2. Jiang, C., Yu, L., Tu, Q., Zhao, Y., Zhang, H., Zhao, S. Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-q32.1 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 261-261, 1999.

RPS6KA5

Ribosomal protein S6 kinase alpha-5

ribosomal protein S6 kinase, 90kDa, polypeptide 5

90 kDa ribosomal protein S6 kinase 5 antibody KS6A5_HUMAN antibody MGC1911 antibody Mitogen and stress activated protein kinase 1 antibody MSPK1 antibody Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody Nuclear mitogen- and stress-activated protein kinase 1 antibody Ribosomal protein S6 kinase 90kD polypeptide 5 antibody Ribosomal protein S6 kinase 90kDa antibody Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody Ribosomal Protein S6 Kinase Alpha 5 antibody Ribosomal protein S6 kinase alpha-5 antibody RLPK antibody RPS6KA5 antibody RSK Like Protein Kinase antibody RSK-like protein kinase antibody RSKL antibody S6K alpha 5 antibody S6K-alpha-5 antibody

O75582