Anti-Mitofusin 1 Antibody | Boster Immunoleader PB9264 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Mitofusin 1 Antibody

catalog :

PB9264

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Mitofusin 1 Antibody

Catalog Number :

PB9264

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitofusin-1(MFN1) detection. Tested with WB in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for Mitofusin 1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 8090 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5. MFN1 has two key domains. One is a coiledcoil region that mediates MFN1: MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Reference :

1. Adams MD, Kerlavage AR, Fields C, Venter JC (Sep 1993). "3,400 new expressed sequence tags identify diversity of transcripts in human brain". Nat Genet 4 (3): 256–67. 2. Meeusen, S., McCaffery, J. M., Nunnari, J. Mitochondrial fusion intermediates revealed in vitro. Science 305: 1747-1752, 2004. 3. Santel, A., Fuller, M. T. Control of mitochondrial morphology by a human mitofusin. J. Cell Sci. 114: 867-874, 2001.

Gene Name :

MFN1

Protein Name :

Mitofusin-1

Gene Full Name :

mitofusin 1

Synonyms :

Fzo homolog antibody Hfzo1 antibody Hfzo2 antibody MFN 1 antibody Mfn1 antibody MFN1_HUMAN antibody Mitochondrial transmembrane GTPase Fzo 1 antibody Mitochondrial transmembrane GTPase FZO 2 antibody Mitochondrial transmembrane GTPaseFZO1B antibody Mitofusin 1 antibody Mitofusin-1 antibody Mitofusin1 antibody Putative transmembrane GTPase antibody Transmembrane GTPase MFN1 antibody

Uniprot ID :

Q8IWA4

more info or order :

Boster Immunoleader product webpage