Anti-MAD1 Antibody | Boster Immunoleader PB9262 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MAD1 Antibody

catalog :

PB9262

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-MAD1 Antibody

Catalog Number :

PB9262

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD1(MAD1L1) detection. Tested with WB, IHC-P in Human;Mouse;Rat

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for MAD1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. It is mapped to 7p22.3. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 can function as a homodimer. It localizes to the centrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase. MAD1L1 may also play a role in cell cycle control and tumor suppression.

Reference :

1. "Entrez Gene: MAD1L1 MAD1 mitotic arrest deficient-like 1 (yeast)" 2. Jin, D.-Y., Kozak, C. A., Pangilinan, F., Spencer, F., Green, E. D., Jeang, K.-T. Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5. Genomics 55: 363-364, 1999. 3. Jin, D.-Y., Spencer, F., Jeang, K.-T. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell 93: 81-91, 1998. 4. Tsukasaki, K., Miller, C. W., Greenspun, E., Eshaghian, S., Kawabata, H., Fujimoto, T., Tomonaga, M., Sawyers, C., Said, J. W., Koeffler, H. P. Mutations in the mitotic check point gene, MAD1L1, in human cancers. Oncogene 20: 3301-3305, 2001.

Gene Name :

MAD1L1

Protein Name :

Mitotic spindle assembly checkpoint protein MAD1

Gene Full Name :

MAD1 mitotic arrest deficient-like 1 (yeast)

Synonyms :

hMAD1 antibody HsMAD1 antibody MAD1 antibody MAD1 mitotic arrest deficient like 1 (yeast) antibody MAD1-like protein 1 antibody MAD1L1 antibody MD1L1_HUMAN antibody Mitotic arrest deficient 1 antibody Mitotic arrest deficient 1-like protein 1 antibody Mitotic checkpoint MAD1 protein homolog antibody Mitotic spindle assembly checkpoint protein MAD1 antibody PIG9 antibody Tax binding protein 181 antibody Tax-binding protein 181 antibody TP53I9 antibody Tumor protein p53 inducible protein 9 antibody TXBP181 antibody

Uniprot ID :

Q9Y6D9

more info or order :

Boster Immunoleader product webpage