Anti-MCM7 Antibody | Boster Immunoleader PB9261 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MCM7 Antibody

catalog :

PB9261

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-MCM7 Antibody

Catalog Number :

PB9261

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM7(MCM7) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for MCM7 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

Reference :

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

Gene Name :

MCM7

Protein Name :

DNA replication licensing factor MCM7

Gene Full Name :

minichromosome maintenance complex component 7

Synonyms :

CDABP0042 antibody CDC 47 antibody CDC47 antibody CDC47 homolog antibody Cdc47, S. cerevisiae, homolog of antibody DNA replication licensing factor MCM7 antibody Homolog of S. cerevisiae Cdc47 antibody MCM 2 antibody MCM 7 antibody MCM2 antibody MCM2, formerly antibody Mcm7 antibody MCM7 minichromosome maintenance deficient 7 antibody MCM7_HUMAN antibody Minichromosome Maintainence 7 antibody Minichromosome maintainence, S. cerevisiae, homolog of antibody Minichromosome maintenance complex component 7 antibody Minichromosome maintenance deficient 7 antibody Minichromosome maintenance protein 7 antibody P1.1 MCM3 antibody P1.1-MCM3 antibody P1CDC47 antibody P85MCM antibody PNAS 146 antibody PNAS146 antibody

Uniprot ID :

P33993

more info or order :

Boster Immunoleader product webpage