Boster Immunoleader

antibody

Anti-c-Kit Antibody

PB9258

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-c-Kit Antibody

PB9258

Polyclonal

Rabbit IgG polyclonal antibody for Mast/stem cell growth factor receptor Kit(KIT) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for c-Kit is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

SCFR(Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

1. Barker, P. E., Besmer, P., Ruddle, F. H. Human c-kit oncogene on human chromosome 4. (Abstract) Am. J. Hum. Genet. 37: A143, 1985. 2. Chen, L. L., Sabripour, M., Wu, E. F., Prieto, V. G., Fuller, G. N., Frazier, M. L. A mutation-created novel intra-exonic pre-mRNA splice site causes constitutive activation of KIT in human gastrointestinal stromal tumors. Oncogene 24: 4271-4280, 2005. 3. Andre C, Hampe A, Lachaume P, Martin E, Wang XP, Manus V, Hu WX, Galibert F (January 1997). "Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes". Genomics 39(2): 216–26. 4. Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U, Ullrich A (November 1987). "Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand". EMBO J. 6 (11): 3341–51.

KIT

Mast/stem cell growth factor receptor Kit

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

C Kit antibody c-Kit antibody CD 117 antibody CD117 antibody CD117 antigen antibody KIT antibody KIT oncogene antibody KIT_HUMAN antibody Mast cell growth factor receptor antibody Mast/stem cell growth factor receptor antibody Mast/stem cell growth factor receptor Kit antibody p145 c-kit antibody PBT antibody Piebald trait protein antibody Proto oncogene c Kit antibody Proto oncogene tyrosine protein kinase Kit antibody Proto-oncogene c-Kit antibody SCF Receptor antibody SCFR antibody soluble KIT variant 1 antibody Steel Factor Receptor antibody Stem cell factor receptor antibody tyrosine protein kinase Kit antibody Tyrosine-protein kinase Kit antibody v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody

P10721