Anti-Fas Antibody | Boster Immunoleader PB9252 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Fas Antibody

catalog :

PB9252

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, et al. Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis. Osteoarthritis Cartilage. 2014;22:1197-205 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Fas Antibody

Catalog Number :

PB9252

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Fas is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Reference :

1. Desbarats, J.; Birge, R. B.; Mimouni-Rongy, M.; Weinstein, D. E.; Palerme, J.-S.; Newell, M. K. : Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nature Cell Biol. 5: 118-125, 2003. 2. Arscott, P. L.; Stokes, T.; Myc, A.; Giordano, T. J.; Thompson, N. W.; Baker, J. R., Jr. : Fas (CD95) expression is up-regulated on papillary thyroid carcinoma. J. Clin. Endocr. Metab. 84: 4246-4252, 1999. 3. Zhang, X.; Miao, X.; Sun, T.; Tan, W.; Qu, S.; Xiong, P.; Zhou, Y.; Lin, D. : Functional polymorphisms in cell death pathway genes FAS and FASL contribute to the risk of lung cancer. J. Med. Genet. 42: 479-484, 2005.

Gene Name :

FAS

Protein Name :

Tumor necrosis factor receptor superfamily member 6

Gene Full Name :

Fas cell surface death receptor

Synonyms :

ALPS 1A antibody ALPS1A antibody APO 1 antibody Apo 1 antigen antibody APO 1 cell surface antigen antibody Apo-1 antigen antibody APO1 antibody Apo1 antigen antibody APO1 cell surface antigen antibody Apoptosis antigen 1 antibody Apoptosis mediating surface antigen FAS antibody Apoptosis-mediating surface antigen FAS antibody APT 1 antibody APT1 antibody CD 95 antibody CD 95 antigen antibody CD95 antibody CD95 antigen antibody Delta Fas antibody Delta Fas/APO 1/CD95 antibody Delta Fas/APO1/CD95 antibody Fas (TNF receptor superfamily, member 6) antibody FAS 1 antibody FAS 827dupA antibody Fas AMA antibody FAS antibody FAS Antigen antibody Fas cell surface death receptor antibody FAS1 antibody FASLG receptor antibody FASTM antibody sFAS antibody Surface antigen APO1 antibody TNF receptor superfamily, member 6 antibody TNFRSF 6 antibody TNFRSF6 antibody TNR6_HUMAN antibody Tumor necrosis factor receptor superfamily member 6 antibody

Uniprot ID :

P25445

more info or order :

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