Anti-Calretinin Antibody | Boster Immunoleader PB9248 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Calretinin Antibody

catalog :

PB9248

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Calretinin Antibody

Catalog Number :

PB9248

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Calretinin(CALB2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminal of human Calretinin(234-262aa EMNIQQLTNYRKSVMSLAEAGKLYRKDLE), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Calretinin, also known as 29 kDa calbindin, is a vitamin D-dependent calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Calretinin is mapped to 16q22.2. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers.

Reference :

1. Parmentier M.; "The human calbindins: cDNA and gene cloning.";Adv. Exp. Med. Biol. 255:233-240(1989). 2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.;"The mouse calretinin gene promoter region: structural and functional components.";Brain Res. Mol. Brain Res. 49:175-187(1997). 3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.;"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.";Biochemistry 30:656-662(1991).

Gene Name :

CALB2

Protein Name :

Calretinin

Gene Full Name :

calbindin 2

Synonyms :

29 kDa calbindin antibody CAB 29 antibody CAB29 antibody CAL 2 antibody CAL2 antibody CALB 2 antibody CALB2 antibody CALB2_HUMAN antibody Calbindin 2 29kDa antibody Calbindin 2 antibody Calbindin D29K antibody Calbindin2 antibody Calretinin antibody CR antibody

Uniprot ID :

P22676

more info or order :

Boster Immunoleader product webpage