Anti-RACK1 Antibody | Boster Immunoleader PB9243 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-RACK1 Antibody

catalog :

PB9243

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

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product information

Product Name :

Anti-RACK1 Antibody

Catalog Number :

PB9243

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Guanine nucleotide-binding protein subunit beta-2-like 1(GNB2L1) detection. Tested with WB in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for RACK1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human RACK1 recombinant protein (Position: T2-R317). Human RACK1 shares 100% amino acid (aa) sequence identity with both mouse and rat RACK1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1 is a 32kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. GNB2L1 is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1 is also a mediator of agonist-induced Ca(2+) release. What’s more, GNB2L1 is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of GNB2L1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and GNB2L1 stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.

Reference :

1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Patterson, R. L., van Rossum, D. B., Barrow, R. K., Snyder, S. H. RACK1 binds to inositol 1,4,5-triphosphate receptors and mediates Ca(2+) release. Proc. Nat. Acad. Sci. 101: 2328-2332, 2004. 3. Robles, M. S., Boyault, C., Knutti, D., Padmanabhan, K., Weitz, C. J. Identification of RACK1 and protein kinase C-alpha as integral components of the mammalian circadian clock. Science 327: 463-466, 2010.

Gene Name :

GNB2L1

Protein Name :

Guanine nucleotide-binding protein subunit beta-2-like 1

Gene Full Name :

guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1

Synonyms :

Cell proliferation-inducing gene 21 protein antibody GBLP_HUMAN antibody Gnb2 rs1 antibody Gnb2-rs1 antibody GNB2L1 antibody Gnb2rs1 antibody Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody Guanine nucleotide binding protein beta subunit 2 like 1 antibody Guanine nucleotide binding protein beta subunit like protein 12.3 antibody Guanine nucleotide binding protein subunit beta 2 like 1 antibody Guanine nucleotide binding protein subunit beta like protein 12.3 antibodyGuanine nucleotide-binding protein subunit beta-2-like 1 antibody Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody H12.3 antibody HLC 7 antibody HLC-7 antibody HLC7 antibody Human lung cancer oncogene 7 protein antibody Lung cancer oncogene 7 antibody OTTHUMP00000223704 antibody OTTHUMP00000223870 antibody OTTHUMP00000223891 antibody OTTHUMP00000223893 antibody OTTHUMP00000223900 antibody OTTHUMP00000223902 antibody OTTHUMP00000223930 antibody OTTHUMP00000223931 antibody PIG 21 antibody PIG21 antibody Proliferation inducing gene 21 antibody Protein homologous to chicken B complex protein guanine nucleotide binding antibody RACK 1 antibody RACK1 antibody Receptor for activated C kinase antibody Receptor for activated C kinase 1 antibody Receptor of activated protein kinase C 1 antibody

Uniprot ID :

P63244

more info or order :

Boster Immunoleader product webpage