Anti-Hsp27 Antibody | Boster Immunoleader PB9237 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hsp27 Antibody

catalog :

PB9237

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Hsp27 Antibody

Catalog Number :

PB9237

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Hsp27 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

HSPB1(Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Reference :

1. Ackerley, S., James, P. A., Kalli, A., French, S., Davies, K. E., Talbot, K. A mutation in the small heat-shock protein HSPB1 leading to distal hereditary motor neuronopathy disrupts neurofilament assembly and the axonal transport of specific cellular cargoes. Hum. Molec. Genet. 15: 347-354, 2006. 2. d'Ydewalle, C., Krishnan, J., Chiheb, D. M., Van Damme, P., Irobi, J., Kozikowski, A. P., Vanden Berghe, P., Timmerman, V., Robberecht, W., Van Den Bosch, L. HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1-induced Charcot-Marie-Tooth disease. Nature Med. 17: 968-974, 2011. 3. Stock, A. D., Spallone, P. A., Dennis, T. R., Netski, D., Morris, C. A., Mervis, C. B., Hobart, H. H. Heat shock protein 27 gene: chromosomal and molecular location and relationship to Williams syndrome. Am. J. Med. Genet. 120A: 320-325, 2003.

Gene Name :

HSPB1

Protein Name :

Heat shock protein beta-1

Gene Full Name :

heat shock 27kDa protein 1

Synonyms :

Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody Heat Shock Protein27 antibody Heat Shock Protein27 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody Hsp 25 antibody Hsp 27 antibody Hsp 27 antibody Hsp 28 antibody Hsp 28 antibody Hsp B1 antibody Hsp B1 antibody Hsp25 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody Hsp28 antibody HspB1 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 27 antibody

Uniprot ID :

P04792

more info or order :

Boster Immunoleader product webpage