Boster Immunoleader

antibody

Anti-ZEB2 Antibody

PB9236

100 ug/vial

240 USD

polyclonal

rabbit

Anti-ZEB2 Antibody

PB9236

Polyclonal

Rabbit IgG polyclonal antibody for Zinc finger E-box-binding homeobox 2(ZEB2) detection. Tested with WB in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human

WB: The detection limit for ZEB2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

ZEB2(Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B(ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.

1. Beltran, M., Puig, I., Pena, C., Garcia, J. M., Alvarez, A. B., Pena, R., Bonilla, F., Garcia de Herreros, A. A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev. 22: 756-769, 2008. 2. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. 3. Vandewalle, C., Comijn, J., De Craene, B., Vermassen, P., Bruyneel, E., Andersen, H., Tulchinsky, E., Van Roy, F., Berx, G. SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. Nucleic Acids Res. 33: 6566-6578, 2005. 4. Wakamatsu, N., Yamada, Y., Yamada, K., Ono, T., Nomura, N., Taniguchi, H., Kitoh, H., Mutoh, N., Yamanaka, T., Mushiake, K., Kato, K., Sonta, S., Nagaya, M. Mutations in SIP1, encoding Smad interacting protein-1, cause a form of Hirschsprung disease. Nature Genet. 27: 369-370, 2001.

ZEB2

Zinc finger E-box-binding homeobox 2

zinc finger E-box binding homeobox 2

FLJ42816 antibody HSPC082 antibody KIAA0569 antibody SIP 1 antibody SIP1 antibody Smad-interacting protein 1 antibody SMADIP 1 antibody SMADIP1 antibody ZEB 2 antibody ZEB2 antibody ZEB2_HUMAN antibody Zfhx1b antibody ZFHX1B protein antibody Zfx1b antibody Zinc finger E box binding protein 2 antibody Zinc finger E-box-binding homeobox 2 antibody Zinc finger homeobox 1b antibody zinc finger homeobox protein 1 antibody Zinc finger homeobox protein 1b antibody

O60315