Anti-JunB Antibody | Boster Immunoleader PB9226 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-JunB Antibody

catalog :

PB9226

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-JunB Antibody

Catalog Number :

PB9226

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcription factor jun-B(JUNB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for JunB is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human JunB (323-347aa QLKQKVMTHVSNGCQLLLGVKGHAF), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene. It is mapped to 19p13.2. JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50%) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene. What's more, the expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.

Reference :

1. Schütte J, Viallet J, Nau M, Segal S, Fedorko J, Minna J (Feb 1990). "jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun". Cell 59 (6): 987–997. 2. Mollinedo F, Vaquerizo MJ, Naranjo JR (1991). "Expression of c-jun, jun B and jun D proto-oncogenes in human peripheral-blood granulocytes". Biochem. J. 273(Pt 2): 477–9. 3. Phinney DG, Tseng SW, Ryder K (1996). "Complex genetic organization of junB: multiple blocks of flanking evolutionarily conserved sequence at the murine and human junB loci". Genomics 28 (2): 228–234.

Gene Name :

JUNB

Protein Name :

Transcription factor jun-B

Gene Full Name :

jun B proto-oncogene

Synonyms :

Activator protein 1 antibody AP 1 antibody AP1 antibody Jun B antibody Jun B proto oncogene antibody Jun B protooncogene antibody Junb antibody JunB proto oncogene antibody JunB protoncogene 9 antibody JunB protooncogene antibody JUNB_HUMAN antibody Transcription factor jun B antibody Transcription factor jun-B antibody Transcription factor junB antibody

Uniprot ID :

P17275

more info or order :

Boster Immunoleader product webpage