Boster Immunoleader

antibody

Anti-HNF-4-alpha Antibody

PB9215

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

info changed 20150417

Anti-HNF-4-alpha Antibody

PB9215

Polyclonal

Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 4-alpha(HNF4A) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

WB: The detection limit for HNF-4-alpha is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

1. Chartier FL, Bossu JP, Laudet V, Fruchart JC, Laine B (September 1994). "Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver". Gene 147 (2): 269–72. 2. Argyrokastritis A, Kamakari S, Kapsetaki M, Kritis A, Talianidis I, Moschonas NK (February 1997). "Human hepatocyte nuclear factor-4 (hHNF-4) gene maps to 20q12-q13.1 between PLCG1 and D20S17". Hum. Genet. 99 (2): 233–6. 3. Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB (February 2003). "The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4". Nat. Med. 9 (2): 220–4.

HNF4A

Hepatocyte nuclear factor 4-alpha

hepatocyte nuclear factor 4, alpha

FLJ39654 antibody Hepatic nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 alpha antibody Hepatocyte nuclear factor 4 antibody Hepatocyte nuclear factor 4-alpha antibody HNF 4 alpha antibody HNF 4 antibody HNF-4-alpha antibody HNF4 antibody HNF4 antibody HNF4A antibody HNF4A_HUMAN antibody HNF4a7 antibody HNF4a8 antibody HNF4a9 antibody Hnf4alpha antibody HNF4alpha10/11/12 antibody MODY 1 antibody MODY antibody MODY1 antibody NR2A1 antibody NR2A1 antibody NR2A21 antibody Nuclear receptor subfamily 2 group A member 1 antibody OTTHUMP00000031060 antibody OTTHUMP00000031062 antibody TCF 14 antibody TCF antibody TCF-14 antibody TCF14 antibody TCF14 antibody Tcf4 antibody Transcription factor 14, hepatic nuclear factor antibody Transcription factor 14 antibody Transcription factor HNF 4 antibody Transcription factor HNF-4 antibody Transcription factor HNF4 antibody

P41235