Boster Immunoleader

antibody

Anti-GRIA2 Antibody

PB9205

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-GRIA2 Antibody

PB9205

Polyclonal

Rabbit IgG polyclonal antibody for Glutamate receptor 2(GRIA2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat Predicted to work with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

WB: The detection limit for GRIA2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Glutamate receptor 2, also known as GLUR2, is a protein that in humans is encoded by the GRIA2 gene. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. GLUR2's cytogenetic location is 4q32.1. The crystal structures of the GLUR2 ligand-binding core in the apo state and in the presence of the antagonist DNQX, the partial agonist kainate, and the full agonists AMPA and glutamate.GLUR2 plays a major role in depression at synapses in which glutamate remains in the synaptic cleft for prolonged periods of time during normal operation of the synapse. The overexpression of GLUR2 increases dendritic spine size and density in hippocampal neurons, and more remarkably, induces spine formation in GABA-releasing interneurons that normally lack spines.

1. Armstrong, N., Gouaux, E. Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core. Neuron 28: 165-181, 2000. 2. Biou, V., Bhattacharyya, S., Malenka, R. C. Endocytosis and recycling of AMPA receptors lacking GluR2/3. Proc. Nat. Acad. Sci. 105: 1038-1043, 2008. 3. Mameli, M., Balland, B., Lujan, R., Luscher, C. Rapid synthesis and synaptic insertion of GluR2 for mGluR-LTD in the ventral tegmental area. Science 317: 530-533, 2007.

GRIA2

Glutamate receptor 2

glutamate receptor, ionotropic, AMPA 2

AMPA 2 antibody AMPA selective glutamate receptor 2 antibody AMPA-selective glutamate receptor 2 antibody AMPA2 antibody GluA2 antibody GLUR 2 antibody GLUR B antibody GluR K2 antibody GluR-2 antibody GluR-B antibody GluR-K2 antibody GLUR2 antibody GLURB antibody Glutamate receptor 2 antibody Glutamate receptor ionotropic AMPA 2 antibody Glutamate receptor ionotropic antibody Gria2 antibody GRIA2_HUMAN antibody HBGR2 antibody

P42262