Anti-GCLC Antibody | Boster Immunoleader PB9201 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GCLC Antibody

catalog :

PB9201

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GCLC Antibody

Catalog Number :

PB9201

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for GCLC is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Reference :

1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

Gene Name :

GCLC

Protein Name :

Glutamate--cysteine ligase catalytic subunit

Gene Full Name :

glutamate-cysteine ligase, catalytic subunit

Synonyms :

EC 6.3.2.2 antibody Gamma ECS antibody Gamma GCS antibody Gamma glutamylcysteine synthetase antibody Gamma-ECS antibody Gamma-glutamylcysteine synthetase antibody GCL antibody GCLC antibody GCLM antibody GCS antibody GCS Heavy Chain antibody GLCL antibody GLCLC antibody GLCLR antibody Glutamate cysteine ligase catalytic subunit antibody Glutamate cysteine ligase (gamma glutamylcysteine synthetase) regulatory (30.8kD) antibody Glutamate cysteine ligase catalytic subunit antibody Glutamate cysteine ligase modifier subunit antibody Glutamate cysteine ligase regulatory protein antibody Glutamate cysteine ligase regulatory subunit antibody Glutamate--cysteine ligase catalytic subunit antibody GSC light chain antibody GSH1_HUMAN antibody

Uniprot ID :

P48506

more info or order :

Boster Immunoleader product webpage