Anti-FOXO1A Antibody | Boster Immunoleader PB9195 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-FOXO1A Antibody

catalog :

PB9195

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-FOXO1A Antibody

Catalog Number :

PB9195

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Forkhead box protein O1(FOXO1) detection. Tested with WB in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Notes :

WB: The detection limit for FOXO1A is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Forkhead box protein O1, also known as FKHR or FOXO1A, is a protein that in humans is encoded by the FOXO1 gene. It is mapped to 13q14.11. This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO1 plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. This gene is primarily regulated through phosphorylation on multiple residues. Its transcriptional activity is dependent on its phosphorylation state. What's more, FOXO1 may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Reference :

1. Galili N, Davis RJ, Fredericks WJ, Mukhopadhyay S, Rauscher FJ, Emanuel BS, Rovera G, Barr FG (November 1993). "Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma". Nat. Genet. 5 (3): 230–5. doi:10.1038/ng1193-230 2. Nakae J, Kitamura T, Kitamura Y, Biggs WH, Arden KC, Accili D (January 2003). "The forkhead transcription factor Foxo1 regulates adipocyte differentiation". Dev. Cell 4 (1): 119–29. 3. Rena G, Guo S, Cichy SC, Unterman TG, Cohen P (June 1999). "Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B". J. Biol. Chem. 274 (24): 17179–83.

Gene Name :

FOXO1

Protein Name :

Forkhead box protein O1

Gene Full Name :

forkhead box O1

Synonyms :

FKH 1 antibody FKH1 antibody FKHR antibody Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody Forkhead box O1 antibody Forkhead box protein O1 antibody Forkhead box protein O1A antibody Forkhead in rhabdomyosarcoma antibody Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody FoxO transcription factor antibody foxo1 antibody FOXO1_HUMAN antibody FOXO1A antibody OTTHUMP00000018301 antibody

Uniprot ID :

Q12778

more info or order :

Boster Immunoleader product webpage