Anti-CYP2E1 Antibody | Boster Immunoleader PB9190 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CYP2E1 Antibody

catalog :

PB9190

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-CYP2E1 Antibody

Catalog Number :

PB9190

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Notes :

WB: The detection limit for CYP2E1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Reference :

1. Kölble K (December 1993). "Regional mapping of short tandem repeats on human chromosome 10: cytochrome P450 gene CYP2E, D10S196, D10S220, and D10S225". Genomics 18 (3): 702–4. 2. Hayashi S, Watanabe J, Kawajiri K (October 1991). "Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene". J. Biochem. 110 (4): 559–65. 3. Glew, Robert H. "You Can Get There From Here: Acetone, Anionic Ketones and Even-Carbon Fatty Acids can Provide Substrates for Gluconeogenesis". Retrieved August 2013.

Gene Name :

CYP2E1

Protein Name :

Cytochrome P450 2E1

Gene Full Name :

cytochrome P450, family 2, subfamily E, polypeptide 1

Synonyms :

4-nitrophenol 2-hydroxylase antibody CP2E1_HUMAN antibody CPE 1 antibody CPE1 antibody CYP2E antibody CYP2E1 antibody CYPIIE1 antibody Cytochrome P450 2E1 antibody cytochrome P450 family 2 subfamily E polypeptide 1 antibody Cytochrome P450 isozyme 3A antibody Cytochrome P450 LM3A antibody Cytochrome P450 subfamily IIE (ethanol inducible) polypeptide 1 antibody Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody Cytochrome P450, subfamily IIE antibody Cytochrome P450-ALC antibody Cytochrome P450-J antibody Cytochrome P450RLM6 antibody EC 1.14.13.- antibody EC 1.14.13.n7 antibody Ethanol inducible p450 antibody Ethanol-inducible P450 antibody flavoprotein-linked monooxygenase antibody MGC133529 antibody microsomal monooxygenase antibody OTTHUMP00000020813 antibody OTTHUMP00000046571 antibody P450 ALC antibody P450 J antibody P450-J antibody P450C2E antibody P450J antibody P450RLM6 antibody xenobiotic monooxygenase antibody

Uniprot ID :

P05181

more info or order :

Boster Immunoleader product webpage